| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schmidt, Kathryn R (12)
|
MDCAC |
42.61 |
40.89 |
-1.72 |
| 2 |
Milani, Ashlyn K (11)
|
MDSMDA |
44.91 |
42.02 |
-2.89 |
| 3 |
Reynolds, Ashley L (11)
|
MDCAC |
44.93 |
44.55 |
-0.38 |
| 4 |
Sims, Aaliyah R (11)
|
MDSMDA |
46.37 |
45.22 |
-1.15 |
| 5 |
Eversole, Jayden M (11)
|
MDCAC |
44.95 |
45.51 |
0.56 |
| 6 |
Hahn, Jean (11)
|
MDSPRC |
46.42 |
45.72 |
-0.70 |
| 7 |
Ivall, Olivia P (11)
|
MDFAST |
45.55 |
46.65 |
1.10 |
| 8 |
Burns, Madelyn A (12)
|
MDFAST |
46.04 |
47.45 |
1.41 |
| 9 |
de la Cerda, Anastasia M (11)
|
MDCAC |
49.45 |
49.23 |
-0.22 |
| 10 |
Mason, Lilly K (11)
|
MDCBAC |
51.89 |
51.24 |
-0.65 |
| 11 |
Cost, Sydney F (11)
|
MDCAC |
52.52 |
52.36 |
-0.16 |
| 12 |
Edwards, Emily M (11)
|
MDASC |
1:04.97 |
59.94 |
-5.03 |
| 13 |
Stay, Madelyn C (11)
|
MDCAC |
|
1:04.57 |
- |
| 14 |
Cortes, Chrystelle Aice B (12)
|
MDCAC |
|
1:04.91 |
- |
|
Arias, Mia F (11)
|
MDCAC |
1:04.69 |
NS |
- |